Undetermined_S0_L001_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	Undetermined_S0_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34933991
Sequences flagged as poor quality	0
Sequence length	50
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	2238312	6.40726105414065	No Hit
CCTATCCTTGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTC	2003343	5.734652533688464	No Hit
GGCTCTGATGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTC	1397026	3.999044941644372	No Hit
CCTATCCTGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTCC	1143757	3.2740519112173585	No Hit
GGCTCTGAGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTCC	1035754	2.9648888384954355	No Hit
ATAGAGGCTGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTC	486493	1.3926064159116547	No Hit
ATAGAGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	444408	1.272136355677197	RNA PCR Primer, Index 31 (96% over 28bp)
TATAGCCTTGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTC	324901	0.9300426052093503	No Hit
ATAGAGGCGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTCC	288993	0.8272544639975432	No Hit
TATAGCCTGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTCC	252642	0.7231982168885313	No Hit
ATAGAGGCATACAAATTCCTCGTAGGCGCTATCTCGTATGCCGTCTTCTG	128866	0.3688842766347538	RNA PCR Primer, Index 31 (95% over 24bp)
GGCTCTGAGGGAGCCATCAAGATGTCAAAAAATCCTTGAGGATTGGATGC	67140	0.1921910382355111	No Hit
TATAGCCTCGTAGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	66014	0.18896781647421848	RNA PCR Primer, Index 31 (96% over 28bp)
GGCTCTGACCCATTGAACGGAATAACAGGTTGGATGATAAGTCCCCGGTC	60782	0.17399099919616973	No Hit
CCTATCCTGGGAGCCATCAAGATGTCAAAAAATCCTTGAGGATTGGATGC	57230	0.16382325168630174	No Hit
GACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTCCTCGTAGGC	53558	0.1533119991929923	No Hit
GGCTCTGACCAAGCATCGAAAGATAACAGGTTGGATGATAAGTCCCCGGT	52400	0.14999717610278196	No Hit
CCTATCCTCGTAGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	50154	0.14356790782936882	TruSeq Adapter, Index 25 (96% over 27bp)
ATAGAGGCGGGAGCCATCAAGATGTCAAAAAATCCTTGAGGATTGGATGC	48460	0.1387187624797865	No Hit
TATAGCCTGGGAGCCATCAAGATGTCAAAAAATCCTTGAGGATTGGATGC	47864	0.13701268772869382	No Hit
GGCTCTGACACATCTCCCTATAGTGAGTCGTATTACGATACAAATTCCTC	41841	0.11977160010145992	No Hit
ATAGAGGCGACTATATTGGTACCTAACAGGTTGGATGATAAGTCCCCGGT	37456	0.10721935549820232	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ATAGAGG	755270	43.976185	1
GGCTCTG	735820	43.96719	1
TAGAGGC	756130	43.921497	2
TATAGCC	569230	43.915897	1
AGAGGCG	278250	43.907944	3
GCTCTGA	738200	43.894657	2
AGAGGCA	152060	43.83315	3
CCTATCC	889220	43.78856	1
GAGGCGC	98280	43.760204	4
AGAGGCC	150710	43.738155	3
ATAGCCT	579500	43.714413	2
TATCCTG	278355	43.696926	3
GAGGCGA	86680	43.686634	4
GAGACAC	109555	43.67989	7
CTATCCT	892470	43.67385	2
GAGGCCC	43980	43.65126	4
GGCGACA	35515	43.634373	6
CTCTGAG	254545	43.613483	3
AGGCGCC	14875	43.591797	5
GAGGCGT	55855	43.584965	4

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content